|
|
Yeasts and wine
Palíková, Petra ; Vadkertiová, Renata (referee) ; Vránová, Dana (advisor)
This thesis deals with isolation and identification wine yeasts from grapes and must. For analysis was used white wine Sauvignon that was grown and producing after needs ecological agriculture. Remove samples were processed in laboratory and by the help of dilution method were obtained pure culture isolated yeasts. In the following step, by the application of commercial kit UltraCleanTM Microbial DNA Isolation Kit we were able to isolated individual DNA that it was used to the next analysis. Isolated DNA was amplification by PCR method with ITS1 and ITS4 primers. PCR products were detected on agarose gel. Amplification samples were chopped five restriction endonucleases: HaeIII, HinfI, TaqaI, AluI and MseI. Chopped DNA was detected by the same way as PCR products and it was compared with restriction patterns of collection yeasts. In the next step it was compared genetic similarity of isolated yeasts by using BioNumerics software. As a criterion it was used Pearson coefficients and UPGMA clastering analysis. The result is dedrogram of genetics similarity isolated yeasts.
|
|
|
Laboratory device with temperature control
Telecký, Jakub ; Škrabánek, Pavel (referee) ; Němec, Zdeněk (advisor)
This work deals with regulation and procedures that can be used for this. The theoretical part therefore presents the basic concepts of automation and controllers. The main part is the practical part. The purchased system consists of a regulated system, which must be set up correctly. Using system identification and known procedures, sample examples of how to best set the controller are compiled. The result is a sample laboratory task that automation students can try and gain experience with methods of setting up the controller.
|
|
|
Adaptive controllers with principles of artificial intelligence and its comparison with classical identifications methods
Vaňková, Tereza ; Dokoupil, Jakub (referee) ; Pivoňka, Petr (advisor)
Master’s thesis is focused on the adaptive controllers. The first theoretic part mainly describes the parametric identification, which belongs to the most important part of the adaptive controller’s structure. Classical identification methods (the recursive least squares methods) are firstly mentioned and afterwards the identification methods based on the neural network (the Marquardt-Levenberg algorithm and the new identification algorithm NIA inspired by the neural networks) are described. At the conclusion of the theoretic part there are mentioned the algorithm of the adaptive controller’s tuning which uses the identification parameters (the modified Z-N method) and the tested types of adaptive controllers. Particular results, which were found out by verifying of the adaptive controllers on the simulation and real models, are contained in second, the practical, part of the thesis. Finally, achieved results are compared with the classical discrete PID controller and with the adaptive controller of the B&R company.
|
|
|
Biometry based on retinal videosequences
Oweis, Kamil ; Odstrčilík, Jan (referee) ; Kolář, Radim (advisor)
The biometric methods are the most advanced methods for recognition and verification of person identity. These methods are quite fast, safe and applicable in different situations. In this thesis is used a set of retinal scans taken with a video-ophtalmoscope. These pictures are further modified for next processing, first of all by convertion into black-andwhite binary image, in some cases was after that used a binary matrix for description of image. Afterwards was suggested comparison method of images from the database with reference image of the retina: method of overlap and shift. It was tested a set of blackand-white and then also grey images. All method calculations was realized in program Matlab of which outcome was determination of the most congruent image with reference image and evaluation of overall program accuracy.
|
|
| |
|
|
Jaroslavice – place in the landscape
Májek, Jan ; Veselá, Markéta (referee) ; Foretník, Jan (advisor)
The work is aimed at the center of the village, which is a square that, in size comparable to that of the Square of Freedom in Brno, is an exhibition of problems: considerable deterioration of the building fund, underground use of the public space, which is moreover unreadable and unreachable. The proposed solution divides the area of the square into three smaller public spaces that have a different character and function. Mariánské námětí is the torso of the existing one, which has been earmarked for the building of a new municipal office and library, is characterized by town houses and the location of services and public amenities. The second area is a semi-detached cottage. It is dominated by walnuts and water. The proposed buildings are oriented towards the library and the wine house. The third such defined area is the Wine Market, which is the crossroads, the scattered area of the surrounding houses, like a school, shops, or wine house. A large part of the COOP building has a sports complex with a halo and a swimming pool. There are solved especially the buildings of the town hall with a library and a wine house.
|
|
|
RFID acces system realization
Buchal, Adam ; Matyáš, Pavel (referee) ; Marada, Tomáš (advisor)
This bachelor thesis deals with the use of RFID technology in access control systems in intelligent buildings. The study also describes the principle functions of RFID readers and the list of frequencies at which the technology works. The next section is dedicated to the design of the access system with iClass R10 operating at a frequency of 13.56 MHz, the whole system is controlled by a microprocessor ATmega8. The board design is described program for creating electronic schematics EAGLE. The try-out functionality and basic programming functions used kit EVB 4.3 The last section describes the procedure for the production of printed circuit boards photo path including drawings and schemes and also describes the program controlling the entire device using the block diagram.
|
|
|
Least-Squares Method in System Identification
Matějka, Jiří ; Bělohradský, Petr (referee) ; Máša, Vítězslav (advisor)
This bachelor thesis deals with the description of the least squares method and its application in system identification of dynamic processes. It contains mathematical derivation of least squares method and basic principles of system identification. It describes parametrization of an mathematical model of experimental unit for biomass combustion.
|
|
|
Industrial PID controller with autotuning and visualisation
Vávra, Pavel ; Dvořáček, Martin (referee) ; Pivoňka, Petr (advisor)
This thesis deals with implementation of industrial used controlling block into Power Panel equipment of B&R in integrated development environment Automation Studio 3.0.71. It is PID controller with autotuner and visualization, which allowed bumpless transfer among controlling algorithms and manual control. PID and I-PD controllers with filtering of derivative action and dynamic antiwindup were implemented. Parameters of PID controller is possible to tune with the aid of ultimate gain and ultimate period according rules of Ziegler-Nichols. Ultimate parameters of controlled plant is possible to acquire with the aid of two identification methods, recursive least squares method and relay feedback. Recursive least squares method was implemented with directional forgetting. For relay feedback were used two types of relays: ideal and saturation relay for improving accuracy of searched ultimate values. The whole solution is programmed in ANSI C which Automation Studio supports. Created controller is control with the aid of touchscreen which is integrated in Power Panel. Trends of process values are viewed on the screen too. For comparison adaptive controller by B&R was implemented. This contoller is standardly supplied with Automation Studio in LoopConR library. All created algorithms were first validated on mathematical model of plant and then on real model in laboratory. The first part of thesis deals with theoretic analysis of used methods. The practical realization is described in the second part of this diploma thesis.
|
|
|
Characterization of selected yeast strains from the environment
Bečková, Alena ; Omelková, Jiřina (referee) ; Vránová, Dana (advisor)
The aim of this bachelor thesis deals with identification type-yeasts, which were obtained from the Culture Collection SAV in Bratislava. The purpose is extension of yeast database in laboratory of Faculty of Chemistry, which is used by identification yeasts in samples taken from food or environment. Identification of yeasts was implemented by method PCR-RFLP. Obtained segment is located in area 5,8S-ITS and is defined by using primers ITS1 and ITS4. Restriction analysis was carried out by restriction endonucleasis HaeIII, HinfI, HhaI and TaqI. Chosen species were also tested for presence of extracellular lipasis. In this part of characterisation was used SpiritBlue Agar. The presence of blue colour is lost by activity of lipasis.
|
guest ::
